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The effects of TP on the MMP-2, MMP-9, TIMP-1 and <t>TIMP-2</t> in the renal tissue of aged rats. Bar graphs show MMP-2 mRNA ( a ), MMP-9 mRNA ( b ), TIMP-1 mRNA ( c ) and TIMP-2 mRNA ( d ). ( e ) Western blot study revealed MMP-2, MMP-9, TIMP-1 and TIMP-2 expression in the renal tissue. Bar graphs illustrate the protein expression of MMP-2 ( f ), MMP-9 ( g ), TIMP-1 ( h ) and TIMP-2 ( i ). The asterisks show significant differences (* P < 0.05, ** P < 0.01).
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The effects of TP on the MMP-2, MMP-9, TIMP-1 and <t>TIMP-2</t> in the renal tissue of aged rats. Bar graphs show MMP-2 mRNA ( a ), MMP-9 mRNA ( b ), TIMP-1 mRNA ( c ) and TIMP-2 mRNA ( d ). ( e ) Western blot study revealed MMP-2, MMP-9, TIMP-1 and TIMP-2 expression in the renal tissue. Bar graphs illustrate the protein expression of MMP-2 ( f ), MMP-9 ( g ), TIMP-1 ( h ) and TIMP-2 ( i ). The asterisks show significant differences (* P < 0.05, ** P < 0.01).
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The effects of TP on the MMP-2, MMP-9, TIMP-1 and TIMP-2 in the renal tissue of aged rats. Bar graphs show MMP-2 mRNA ( a ), MMP-9 mRNA ( b ), TIMP-1 mRNA ( c ) and TIMP-2 mRNA ( d ). ( e ) Western blot study revealed MMP-2, MMP-9, TIMP-1 and TIMP-2 expression in the renal tissue. Bar graphs illustrate the protein expression of MMP-2 ( f ), MMP-9 ( g ), TIMP-1 ( h ) and TIMP-2 ( i ). The asterisks show significant differences (* P < 0.05, ** P < 0.01).

Journal: Scientific Reports

Article Title: Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling

doi: 10.1038/s41598-018-29023-3

Figure Lengend Snippet: The effects of TP on the MMP-2, MMP-9, TIMP-1 and TIMP-2 in the renal tissue of aged rats. Bar graphs show MMP-2 mRNA ( a ), MMP-9 mRNA ( b ), TIMP-1 mRNA ( c ) and TIMP-2 mRNA ( d ). ( e ) Western blot study revealed MMP-2, MMP-9, TIMP-1 and TIMP-2 expression in the renal tissue. Bar graphs illustrate the protein expression of MMP-2 ( f ), MMP-9 ( g ), TIMP-1 ( h ) and TIMP-2 ( i ). The asterisks show significant differences (* P < 0.05, ** P < 0.01).

Article Snippet: Membranes were blocked with 5% skimmed milk 1 h at room temperature, and then were probed with mouse monoclonal anti-collagen I antibody (Abcam, ab90395, 1:1000), rabbit polyclonal anti-collagen IV antibody (Abcam, ab6586, 1:1000), rabbit monoclonal anti-fibronectin antibody (Abcam, ab45688, 1:5000), rabbit polyclonal anti-MMP-2 antibody (Abcam, ab37150, 1:200), rabbit polyclonal anti-MMP-9 antibody (Abcam, ab38898, 1:1000), rabbit polyclonal anti-TIMP-1 antibody (Abcam, ab61224, 1:500), rabbit polyclonal anti-TIMP-2 antibody (Abcam, ab180630, 1:500), goat polyclonal anti-TGF-β1 antibody (Santa-Cruz, C-16, sc-31609, 1:500), mouse monoclonal anti-Smad2/3 antibody (Santa-Cruz, A-3, sc-398844, 1:200), goat polyclonal anti-p-Smad2/3 antibody (Santa-Cruz, Ser 423/425, sc-11769, 1:200) and goat polyclonal anti-Smad 4 antibody (Santa-Cruz, C-20, sc-1909, 1:1000) overnight (4 °C).

Techniques: Western Blot, Expressing